AgrobacTransV1, Main, Exploration, bibRecord, 000262

Transient Expression of Viral Proteins in Plants Using Agrobacterium tumefaciens.

Identifieur interne : 000262 ( Main/Exploration ); précédent : 000261; suivant : 000263

Transient Expression of Viral Proteins in Plants Using Agrobacterium tumefaciens.

Auteurs : Inga I. Hitzeroth [Afrique du Sud] ; Albertha R. Van Zyl [Afrique du Sud]

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.

RBID : pubmed:27076324

Descripteurs français

KwdFr :
- ADN recombiné (génétique), Agrobacterium tumefaciens (génétique), Expression des gènes (MeSH), Facteurs temps (MeSH), Feuilles de plante (composition chimique), Génie génétique (méthodes), Protéines virales (génétique), Protéines virales (isolement et purification), Tabac (génétique), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
MESH :
- composition chimique : Feuilles de plante.
- génétique : ADN recombiné, Agrobacterium tumefaciens, Protéines virales, Tabac.
- isolement et purification : Protéines virales.
- méthodes : Génie génétique.
- Expression des gènes, Facteurs temps, Transformation génétique, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Agrobacterium tumefaciens (genetics), DNA, Recombinant (genetics), Gene Expression (MeSH), Genetic Engineering (methods), Plant Leaves (chemistry), Plants, Genetically Modified (MeSH), Time Factors (MeSH), Tobacco (genetics), Transformation, Genetic (MeSH), Viral Proteins (genetics), Viral Proteins (isolation & purification).
MESH :
- chemical , genetics : DNA, Recombinant, Viral Proteins.
- chemistry : Plant Leaves.
- genetics : Agrobacterium tumefaciens, Tobacco.
- chemical , isolation & purification : Viral Proteins.
- methods : Genetic Engineering.
- Gene Expression, Plants, Genetically Modified, Time Factors, Transformation, Genetic.

Abstract

Transient expression of viral proteins in plants is a novel alternative to other expression platforms. The viral proteins can be used as potential vaccines or in diagnostics. Nicotiana benthamiana leaves or whole plants are infiltrated with recombinant Agrobacterium that harbor the gene of interest. Protein expression in the plants is rapid and results are obtained within 2-7 days. Here we describe how to make electrocompetent Agrobacterium, how to transform Agrobacterium, how to infiltrate leaves or plants with the recombinant Agrobacterium, and lastly how to extract the protein for analysis by gel electrophoresis.

DOI: 10.1007/978-1-4939-3389-1_38
PubMed: 27076324

Affiliations:

Afrique du Sud

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Le document en format XML

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<term>Genetic Engineering (methods)</term>
<term>Plant Leaves (chemistry)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Time Factors (MeSH)</term>
<term>Tobacco (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
<term>Viral Proteins (genetics)</term>
<term>Viral Proteins (isolation & purification)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (génétique)</term>
<term>Agrobacterium tumefaciens (génétique)</term>
<term>Expression des gènes (MeSH)</term>
<term>Facteurs temps (MeSH)</term>
<term>Feuilles de plante (composition chimique)</term>
<term>Génie génétique (méthodes)</term>
<term>Protéines virales (génétique)</term>
<term>Protéines virales (isolement et purification)</term>
<term>Tabac (génétique)</term>
<term>Transformation génétique (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Recombinant</term>
<term>Viral Proteins</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term>
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<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Feuilles de plante</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
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<term>Time Factors</term>
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<front><div type="abstract" xml:lang="en">Transient expression of viral proteins in plants is a novel alternative to other expression platforms. The viral proteins can be used as potential vaccines or in diagnostics. Nicotiana benthamiana leaves or whole plants are infiltrated with recombinant Agrobacterium that harbor the gene of interest. Protein expression in the plants is rapid and results are obtained within 2-7 days. Here we describe how to make electrocompetent Agrobacterium, how to transform Agrobacterium, how to infiltrate leaves or plants with the recombinant Agrobacterium, and lastly how to extract the protein for analysis by gel electrophoresis. </div>
</front>
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<DateRevised><Year>2020</Year>
<Month>11</Month>
<Day>13</Day>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Serveur d'exploration sur l'agrobacterium et la transgénèse

Transient Expression of Viral Proteins in Plants Using Agrobacterium tumefaciens.

Transient Expression of Viral Proteins in Plants Using Agrobacterium tumefaciens.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki